Just to let you know that '1000.exe' is not a virus or trojan in any way, I have tested it on a virtual machine and I have not found any suspicious behaviour, I have also use VirusTotal and to me, there are 2 false positives so I think CalvinWilliams your computer may be infected with something else than this.
January 20, 2016 Subject:
It is possible to play 16 bit games on 32 & 64 bit systems
All you need to do for DOS games is to download a free version of DOSBox or use a Virtual Computer emulation like the free VirtualBox loaded with an older version of Windows.
July 11, 2014 Subject:
Gave Me a Virus
This infected my system with a trojan "1000.exe".
Also, I have found out that 16-bit games are NOT
compatible with newer 32/64-bit PCs, so good luck
trying to play these!